The in vitro effect of 5-FU and Tamoxifen Chemotherapeutics on penthose phosphate pathway enzymes

The pentose phosphate pathway is the metabolic where NADPH, reducing force in metabolism, and ribose 5-phosphate, building block of DNA RNA, are produced. In this study, vitro effects 5-fluorouracil Tamoxifen chemotherapeutic agents on glucose 6-phosphate dehydrogenase (G6PD) 6-phosphogluconate (6PGD), which key enzymes pathway, were investigated. first stage G6PD 6PGD purified from rat erythrocytes using 2ʹ, 5ʹ-ADP Sepharose-4B affinity chromatography. control enzyme activities was determined spectrophotometrically at 340 nm wavelength according to Beutler method. Then, 5-FU agents, widely used chemotherapy, results study showed that increased activity both concentration range 0.19-1.9 mM 26-260 µM.